Hair regeneration potential of human dermal sheath cells cultured under physiological oxygen
We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O2), physiological/normoxic (6% O2), or hypoxic (1% O2) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared to hyperoxia, hypoxia inhibited the proliferation of DPCs but enhanced that of DSCs. In DPCs, hypoxia down-regulated the expression of hair-inductive capacity-related genes, including BMP4, LEF1, SOX2, and VCAN. In DSCs, both normoxia and hypoxia up-regulated SOX2 expression, whereas hypoxia down-regulated BMP4 expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including SPRY, NR0B1, MSX2, IFITM1, and DAZL compared to hyperoxia. In an in vivo hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances the proliferation and maintenance of hair inductivity for skin engineering and clinical applications.
https://www.liebertpub.com/doi/10.1089/ten.TEA.2019.0329
The most interesting part:
„These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances the proliferation and maintenance of hair inductivity for skin engineering and clinical applications.“
But in the end it is still mouse...We don’t know if this will work on humans.
We investigated the effect of oxygen tension on the proliferation and hair-inductive capacity of human dermal papilla cells (DPCs) and dermal sheath cells (DSCs). DPCs and DSCs were separately obtained from human hair follicles and each cultured under atmospheric/hyperoxic (20% O2), physiological/normoxic (6% O2), or hypoxic (1% O2) conditions. Proliferation of DPCs and DSCs was highest under normoxia. Compared to hyperoxia, hypoxia inhibited the proliferation of DPCs but enhanced that of DSCs. In DPCs, hypoxia down-regulated the expression of hair-inductive capacity-related genes, including BMP4, LEF1, SOX2, and VCAN. In DSCs, both normoxia and hypoxia up-regulated SOX2 expression, whereas hypoxia down-regulated BMP4 expression. Microarray analysis revealed that normoxia increased the expression of pluripotency-related genes, including SPRY, NR0B1, MSX2, IFITM1, and DAZL compared to hyperoxia. In an in vivo hair follicle reconstitution assay, cultured DPCs and DSCs were transplanted with newborn mouse epidermal keratinocytes into nude mice using a chamber method. In this experiment, normoxia resulted in the most efficient induction of DPC hair follicles, whereas hypoxia caused the most efficient induction and maturation of DSC hair follicles. These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances the proliferation and maintenance of hair inductivity for skin engineering and clinical applications.
https://www.liebertpub.com/doi/10.1089/ten.TEA.2019.0329
The most interesting part:
„These results suggest that application of physiological/hypoxic oxygen tension to cultured human DSCs enhances the proliferation and maintenance of hair inductivity for skin engineering and clinical applications.“
But in the end it is still mouse...We don’t know if this will work on humans.
