- Reaction score
- 316
Alright guys so I read this patent https://www.google.com/patents/US8841124 that Swoop posted on another thread. I went through like 80% of it, leaving out just the figure descriptions and the tables in the end, since I'm a broscientist and they are way over my head. I was going to post these in the other thread but it would make too big a post so...
First concerning retention of Dermal Papilla Fibroblast properties
"It is particularly the expansion of the isolated DPFs and the physiological administration form that represent limiting factors to the use of these cells in hair growth induction of prior art. It takes several multiplication cycles to achieve the required quantity of cells, during which process the cells cultured in monolayer cultures, especially those of the dermal papilla fibroblasts, lose their inductive abilities, namely, after 5-8 multiplication cycles as shown by experience. The cells thus treated dedifferentiate and express stem cell markers, but can no longer be used to generate hair follicles.
It has been surprisingly demonstrated by the inventors that the cells reach the level of differentiation and stabilization or regain their hair growth-inducing ability after several days to several weeks under the specific culture conditions of the invention, wherein the DPFs expanded following cell culturing are transferred at well-defined concentration into special non-adhesive cell culture vessels. In addition to the regain of inductive properties, it has been surprisingly found that as a result of active cell-cell contacts and exchange of signaling molecules, the cells subsequently form cell aggregates and differentiate. Under these specific conditions, the cells thus treated condense into approximately the shape and size corresponding to the physiological shape of a dermal papilla in a hair follicle following isolation. To this effect, the ratio of cells used/culture vessel surface is of crucial importance."
"As seen from the formation of hair shaft-producing microfollicles, and by means of gene and protein expression analyses, the DPFs reassume their original, inductive properties after specific condensation. Since the inductive properties of cells can be re-established in the course of the present method, the number of passages to expand cells does not matter."
"What can be deduced from table 5 is that with prolonged culture time, the expression level of genes involved in three dimensional arrangement and tissue formation is approaching the expression level observed in native dermal papilla fibroblasts."
Freezing for later use:
"In the course of culturing, the cells can be further expanded for direct use in experiments, or frozen in liquid nitrogen for future use."
Relevant to my question in the Tsuji interview. Inbeforethecure, this one's for you.
"Size and shape of the condensates being formed likewise depend on the region where skin biopsies have been removed. DPFs, which are removed from beard, body hair, eyebrows, genital hair, or head hair, and subjected to expansion form cell aggregates of different size, which in turn determine the respective hair shape, size and length."
This one pertains to their skin equivalent but perhaps the laser can be used on normal scalp for minimum invasion & max density? Totally pulling this thought out of my ***
"and the insertion sites for the microfollicles are cut at regular intervals by means of a 2-photon laser, or pre-perforated with a punch."
Then there's this
"The microorganoid follicles are incorporated into the openings of previously depilated, miniaturized hair follicles (isthmus) of affected skin areas. Preferably, the de novo papillae and hair microfollicles are injected, more preferably by means of a specially constructed device of about 150 μm in size."
It appears that those microfollicles they created don't include sweat glands, nerves or blood vessels. Perhaps these are formed in the skin like with Tsuji's primordia?
Wew. That took some solid time to make as I was on mobile. But it looks like there's another solid crack in the wall of hair loss. Looking forward to the interview. Shadilay bros
First concerning retention of Dermal Papilla Fibroblast properties
"It is particularly the expansion of the isolated DPFs and the physiological administration form that represent limiting factors to the use of these cells in hair growth induction of prior art. It takes several multiplication cycles to achieve the required quantity of cells, during which process the cells cultured in monolayer cultures, especially those of the dermal papilla fibroblasts, lose their inductive abilities, namely, after 5-8 multiplication cycles as shown by experience. The cells thus treated dedifferentiate and express stem cell markers, but can no longer be used to generate hair follicles.
It has been surprisingly demonstrated by the inventors that the cells reach the level of differentiation and stabilization or regain their hair growth-inducing ability after several days to several weeks under the specific culture conditions of the invention, wherein the DPFs expanded following cell culturing are transferred at well-defined concentration into special non-adhesive cell culture vessels. In addition to the regain of inductive properties, it has been surprisingly found that as a result of active cell-cell contacts and exchange of signaling molecules, the cells subsequently form cell aggregates and differentiate. Under these specific conditions, the cells thus treated condense into approximately the shape and size corresponding to the physiological shape of a dermal papilla in a hair follicle following isolation. To this effect, the ratio of cells used/culture vessel surface is of crucial importance."
"As seen from the formation of hair shaft-producing microfollicles, and by means of gene and protein expression analyses, the DPFs reassume their original, inductive properties after specific condensation. Since the inductive properties of cells can be re-established in the course of the present method, the number of passages to expand cells does not matter."
"What can be deduced from table 5 is that with prolonged culture time, the expression level of genes involved in three dimensional arrangement and tissue formation is approaching the expression level observed in native dermal papilla fibroblasts."
Freezing for later use:
"In the course of culturing, the cells can be further expanded for direct use in experiments, or frozen in liquid nitrogen for future use."
Relevant to my question in the Tsuji interview. Inbeforethecure, this one's for you.
"Size and shape of the condensates being formed likewise depend on the region where skin biopsies have been removed. DPFs, which are removed from beard, body hair, eyebrows, genital hair, or head hair, and subjected to expansion form cell aggregates of different size, which in turn determine the respective hair shape, size and length."
This one pertains to their skin equivalent but perhaps the laser can be used on normal scalp for minimum invasion & max density? Totally pulling this thought out of my ***
"and the insertion sites for the microfollicles are cut at regular intervals by means of a 2-photon laser, or pre-perforated with a punch."
Then there's this
"The microorganoid follicles are incorporated into the openings of previously depilated, miniaturized hair follicles (isthmus) of affected skin areas. Preferably, the de novo papillae and hair microfollicles are injected, more preferably by means of a specially constructed device of about 150 μm in size."
It appears that those microfollicles they created don't include sweat glands, nerves or blood vessels. Perhaps these are formed in the skin like with Tsuji's primordia?
Wew. That took some solid time to make as I was on mobile. But it looks like there's another solid crack in the wall of hair loss. Looking forward to the interview. Shadilay bros
