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https://www.ncbi.nlm.nih.gov/pubmed/30068884
Biol Pharm Bull. 2018;41(8):1307-1310. doi: 10.1248/bpb.b18-00283.
Screening of Euphorbiaceae Plant Extracts for Anti-5α-reductase.
Kamei H1, Noguchi K1, Matsuda H1, Murata K1,2.
Author information
Abstract
In our research program to find novel agents for alopecia from natural plant resources, we screened Euphorbiaceae plant extracts using an anti-5α-reductase assay. Among the samples tested, the extract of Phyllanthus urinaria showed the most potent activity with 24.3 and 64.6% inhibition at 50 and 200 µg/mL against the enzyme, respectively. The extract also suppressed the androgen activity of dihydrotestosterone in LNCaP cell line. These results show that the extract of P. urinaria may be a multi-potent agent for androgen-derived alopecia. We tested for activity on a hair regrowth model using mice. The extract of P. urinaria showed hair regrowth activity at 5 mg/mouse/d administration. Furthermore, the active principle for anti-5α-reductase activity was determined as stigmasterol glucoside from activity-guided fractionation and the IC50 was 27.2 µM. These results suggest that extract of P. urinaria may be a promising candidate anti-alopecia agent.
https://www.ncbi.nlm.nih.gov/pubmed/30068884
Biol Pharm Bull. 2018;41(8):1307-1310. doi: 10.1248/bpb.b18-00283.
Screening of Euphorbiaceae Plant Extracts for Anti-5α-reductase.
Kamei H1, Noguchi K1, Matsuda H1, Murata K1,2.
Author information
Abstract
In our research program to find novel agents for alopecia from natural plant resources, we screened Euphorbiaceae plant extracts using an anti-5α-reductase assay. Among the samples tested, the extract of Phyllanthus urinaria showed the most potent activity with 24.3 and 64.6% inhibition at 50 and 200 µg/mL against the enzyme, respectively. The extract also suppressed the androgen activity of dihydrotestosterone in LNCaP cell line. These results show that the extract of P. urinaria may be a multi-potent agent for androgen-derived alopecia. We tested for activity on a hair regrowth model using mice. The extract of P. urinaria showed hair regrowth activity at 5 mg/mouse/d administration. Furthermore, the active principle for anti-5α-reductase activity was determined as stigmasterol glucoside from activity-guided fractionation and the IC50 was 27.2 µM. These results suggest that extract of P. urinaria may be a promising candidate anti-alopecia agent.
