Something weird related to the 2016 Tsuji Team interview with HairLossTalk

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werefckd

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Back then, the Tsuji led research still didn't find a way to multiply epithelial stems cells, a very necessary part for their cloned/bioengineered follicle approach to work, as stated by one of their senior researchers, Koh-ei Toyoshima:

Mr. Toyoshima: For applications of our technology, both mesenchymal stem cells and epithelial stem cells, which are applicable to human clinical applications, are needed. With respect to the development of the technology to cultivate mesenchymal stem cells, that is papilla cells, being applicable to human clinical trials, we are already in progress now for development. On the other hand, as for the technology to cultivate epithelial stem cells of follicles, it still remains a significant challenge globally. We are currently in the middle of research and development for this. Based on our recent research results, we finally have some prospects and expect this issue to be resolved in the near future.

Keiko: That is a huge step toward the clinical application, isn’t it?

Mr. Toyoshima: Yes, in terms of transitioning from basic research to clinical application, we are finally coming out of the basic research phase toward the clinical application phase.
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Keiko: Thank you. Are there any other roadblocks preventing forward progress on this project?

Mr. Toyoshima: Well… at this point, the challenge which I’ve just mentioned is the biggest challenge we face. There are other challenges, but they are rather related to engineering, so in terms of a roadblock for the clinical applications, we believe our issue has been almost solved.
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Clearly, their biggest problem back then was related to the amplification of the epithelial stem cells. And we never had a clear hard confirmation that they ever solved that problem.

Now the weird part: the expansion/multiplication of epithelial stems cells is a problem that was already solved by another scientists back in 2014, as demonstrated in this study: Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells.

From an article related to that discovery:

Dr. Xu says that this “is the first time anyone has made scalable amounts of epithelial stem cells that are capable of generating the epithelial component of hair follicles,” adding that the cells could aid in wound healing, cosmetics and hair regeneration.
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So, yea, I wonder why the Tsuji team couldn't solve a problem that was already solved? Maybe they didn't want to use the same approach used by Dr. Xu for some reason? Dr. Xu used iPSC by the way, the same cells Dr. Terskikh from Stemson is using for the generation of dermal papilla stem cells (and epithelial cells too I presume even though he never addressed that part).
 
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werefckd

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Another weird thing from that interview is that they thought multiplying dermal papilla stem cells was no big deal:

Mr. Toyoshima: For applications of our technology, both mesenchymal stem cells and epithelial stem cells, which are applicable to human clinical applications, are needed. With respect to the development of the technology to cultivate mesenchymal stem cells, that is papilla cells, being applicable to human clinical trials, we are already in progress now for development. On the other hand, as for the technology to cultivate epithelial stem cells of follicles, it still remains a significant challenge globally. We are currently in the middle of research and development for this. Based on our recent research results, we finally have some prospects and expect this issue to be resolved in the near future.
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But Dr. Terskikh stated more than once that until today the multiplication of dermal papilla cells directly (which I believe was Tsuji's approach) is something really hard, hence his method of first multiplying iPSC and then deriving DPSC from them.

www.sbpdiscovery.org

Using stem cells to grow new hair | Sanford Burnham Prebys

www.sbpdiscovery.org www.sbpdiscovery.org
“In adults, dermal papilla cells cannot be readily amplified outside of the body and they quickly lose their hair-inducing properties,” said Terskikh. “We developed a protocol to drive human pluripotent stem cells to differentiate into dermal papilla cells and confirmed their ability to induce hair growth when transplanted into mice.”
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These are the parts of the Tsuji/Organ story that are still not clear and would help explaining what happened that made them shut down a once very promising research. Hopefully the people at Stemson will not repeat the same mistakes!
 
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werefckd

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Selb said:
Have you tried emailing them to ask this?
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Tsuji? No, because I'm nobody and he his very generic and secretive with his responses. Maybe someone with clout in the industry like hairlosstalk or HC2020 could try that.
 
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interesting commet:
"like I said, I think hair cloning works without a doubt but I think the hairs the lab is currently producing are sh*t. The hairs their currently producing look like pubic hair. I’ve read the studies and seen the research. Do I think it will ever get solved. Of course if the money to fund it is there. how long it takes I have no idea. i dont think 2025 is realistic. I see 2030 to be a more appropriate time frame. By then we have quantum computers to run simulations, scientific tooling is magnitudes better. Think about it like this, in the 90’s they didnt have the tools to extract single grafts, it took 10 years to develop the tools before we could start studying the effects of a single graft in other parts of the body. Its more important to focus on creating the instruments than tools to get to it than begging scientists for a cure."
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Cloning whole follicles is one thing. Multiplying & reintroducing DP cells is something else. From what I've read, the DP cell work produces normal hair characteristics.
 
RolfLeeBuckler

RolfLeeBuckler

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In this TV-show Takashi Tsuji told about hair cloning. Is anybody able to view this video and translate it?

www.nhk.jp

ヒューマニエンス 40億年のたくらみ

「ヒューマン」と「サイエンス」の造語「ヒューマニエンス」。人間という不確かで不思議な存在とはいったい何なのか？その真の姿に迫っていくシリーズ。番組ＭＣは、織田裕二さん、ＮＨＫアナウンサーの藤井彩子。
www.nhk.jp www.nhk.jp
 
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werefckd

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werefckd said:
Back then, the Tsuji led research still didn't find a way to multiply epithelial stems cells, a very necessary part for their cloned/bioengineered follicle approach to work, as stated by one of their senior researchers, Koh-ei Toyoshima:




Clearly, their biggest problem back then was related to the amplification of the epithelial stem cells. And we never had a clear hard confirmation that they ever solved that problem.

Now the weird part: the expansion/multiplication of epithelial stems cells is a problem that was already solved by another scientists back in 2014, as demonstrated in this study: Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells.

From an article related to that discovery:



So, yea, I wonder why the Tsuji team couldn't solve a problem that was already solved? Maybe they didn't want to use the same approach used by Dr. Xu for some reason? Dr. Xu used iPSC by the way, the same cells Dr. Terskikh from Stemson is using for the generation of dermal papilla stem cells (and epithelial cells too I presume even though he never addressed that part).
Click to expand...
If you read their words carefully, we never had confirmation of successful induction even for the dermal papilla part. My guess is that they never could properly multiply any of the stems necessary to bio engineer new hairs
 
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