Therapuetic Oglinucliotides Inhibits Androgen Receptor 100%

[Harie]

Sort of strange that I didn't see this posted here too.


Copied from Moximus @ HLH

Silencing the androgen receptor: new skills for antiandrogen oligonucleotide skin and hair therapy.

Dugour A, Hagelin K, Smus C, Balañá ME, Kerner N.

http://uploading.com/files/MCVIRZMD/AR.pdf.html

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This group was able to transfect dermal papilla cells with anti sense anti androgen nucleotides they call ASAO 18.1. In cultures stimulated with DHT they were able to obtain 100% transfection efficience and 100% AR function inhibition. IMO that is unreal, although it would be nice to be tested further.

Unfortunately it is only a letter to the editor and very vague, but promising. A very complicated read though.

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http://www.nature.com/jid/jour.../n5/full/5602805a.html

Antisense oligodeoxynucleotides formulated in cream preparations are being examined in the clinic as topical therapy for psoriasis. To produce their intended anti-inflammatory effects, these large anionic molecules must penetrate the stratum corneum and reach the living epidermis and dermis. A topically applied phosphorothioate antisense oligonucleotide targeted to intercellular adhesion molecule-1 recently was shown to modulate cytokine-inducible target gene expression in engrafted human skin. In this study, we examined the route of entry into mouse skin of fluorochrome-tagged or naked second-generation 2'-O-methoxyethyl-modified oligonucleotides that react specifically with an antibody, using topical cream-based formulations. In hairless mouse skin, immunohistochemical analysis and fluorescence microscopy were unable to detect the presence of oligonucleotide in the epidermis or dermis following topical application although immunostaining was associated with the stratum corneum

 

[somone uk]

well i have to admit i don't know what half the words there mean and i am not willing to google everything i don't know

but from the sounds of this it's a treatment well in it's infancy or complete bollocks but i lack the knowledge to categorise it

it's inevitible that this wouldn't be taken very far though because HM would stunt it's need though improving topicals is always a demanded thing

 

[Harie]

Basically it means that they have made a topical that gets absorbed into the scalp and it inhibited androgen receptors 'locally' by 100%. I'm assuming locally since it didn't mention systematic.

 

[squeegee]

An oligonucleotide is a short nucleic acid polymer, typically with twenty or fewer bases. Although they can be formed by bond cleavage of longer segments, they are now more commonly synthesized by polymerizing individual nucleotide precursors. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases.

The length of the oligonucleotide is usually denoted by "mer" (from Greek meros, "part"). For example, a fragment of 25 bases would be called a 25-mer. Because oligonucleotides readily bind to their respective complementary nucleotide, they are often used as probes for detecting DNA or RNA. Examples of procedures that use oligonucleotides include DNA microarrays, Southern blots, ASO analysis, fluorescent in situ hybridization (FISH), and the synthesis of artificial genes.

Oligonucleotides composed of DNA (oligodeoxyribonucleotides) are often used in the polymerase chain reaction, a procedure that can greatly amplify almost any small piece of DNA. There, the oligonucleotide is referred to as a primer, allowing DNA polymerase to extend the oligonucleotide and replicate the complementary strand.

Antisense therapy is a form of treatment for genetic disorders or infections. When the genetic sequence of a particular gene is known to be causative of a particular disease, it is possible to synthesize a strand of nucleic acid (DNA, RNA or a chemical analogue) that will bind to the messenger RNA (mRNA) produced by that gene and inactivate it, effectively turning that gene "off". This is because mRNA has to be single stranded for it to be translated. Alternatively, the strand might be targeted to bind a splicing site on pre-mRNA and modify the exon content of an mRNA[1].

This synthesized nucleic acid is termed an "anti-sense" oligonucleotide because its base sequence is complementary to the gene's messenger RNA (mRNA), which is called the "sense" sequence (so that a sense segment of mRNA " 5'-AAGGUC-3' " would be blocked by the anti-sense mRNA segment " 3'-UUCCAG-5' ").

Antisense drugs are being researched to treat cancers (including lung cancer, colorectal carcinoma, pancreatic carcinoma, malignant glioma and malignant melanoma), diabetes, ALS, Duchenne muscular dystrophy and diseases such as asthma and arthritis with an inflammatory component. Most potential therapies have not yet produced significant clinical results, though one antisense drug, fomivirsen (marketed as Vitravene), has been approved by the US Food and Drug Administration (FDA) as a treatment for cytomegalovirus retinitis.

THANKS for the good post Harrie! Where can we buy the topical Antisense? I want this motherfucking sh*t!


http://products.invitrogen.com/ivgn/en/ ... ch-Product
http://www.oligofactory.com/products.php
http://www.york-bio.com/OligoOrderForm. ... agodQw7b_Q

Anybody from others forum doing some research to get an hand on it? I wanna be a guinea pig!

 

[hairrific]

100% AR function inhibition! Now that is the topical we have been looking for.

So how many years must go by before we can finally obtain it from CHINA? :sobbing: :dunno:

 

[squeegee]

100% AR function inhibition! Now that is the topical we have been looking for.

So how many years must go by before we can finally obtain it from CHINA? :sobbing: :dunno:

We can get it now.. Just wonder what they used for the delivery agent?

 

[Harie]

Anybody from others forum doing some research to get an hand on it? I wanna be a guinea pig!

Nobody in the other forum was doing more than just sort of discussing it. Mostly how they wondered if something that strong would be systematically absorbed.

The delivery vehicle is definitely something I'd like to know.

EDIT: The only mention of a delivery vehicle was when they talked about using liposomal encapsulation combined with bonding oligonucleotides to lipophilic compounds such as neutral or cationic lipids. So perhaps if they could get their hands on Antisense, Elsom could formulate a custom topical using liposomal or nanosomal encapsulation?

 

[somone uk]

well i can't really get my hopes up on this because most topical anti androgens haven't really delivered on the theory i know this is a different approach in that it inhibits the receptors making hair just like normal but i find the disappointing findings in topical spironolactone and topical finasteride to put me off this
on the other hand the ideal male pattern baldness treatment would be a water soluble androgen receptor inhibitor, then we can mix it into shampoos and make male pattern baldness history

 

[squeegee]

well i can't really get my hopes up on this because most topical anti androgens haven't really delivered on the theory i know this is a different approach in that it inhibits the receptors making hair just like normal but i find the disappointing findings in topical spironolactone and topical finasteride to put me off this
on the other hand the ideal male pattern baldness treatment would be a water soluble androgen receptor inhibitor, then we can mix it into shampoos and make male pattern baldness history

ahahah sorry man but you are so negative!

 

[CCS]

100% AR function inhibition! Now that is the topical we have been looking for.

So how many years must go by before we can finally obtain it from CHINA? :sobbing: :dunno:

Prepare for dry scalp. Hopefully it does not have some other side effect.

 

[TEDDYRUXPIN]

It might be great news but any oligonucleotide started to degrade at room temperate. This so called innovation will have to be stored long term at 4 degree celcius.

The idea and the delivery of transport is there, but it will be very expensive to make. Creating a base pair of oligonucleotide usually costs 23pence per base pair. It will also have to be stored in water or Telogen Effluvium buffer for stability.

Lets wait before people comment on this discovery. Over the 10 years, too much hype and little progress.

Upto now, we can only trust three things:

(1) Finasteride 1mg - DHT inhibitor
(2) Minoxidil - growth stimulator
(3) Hair transplant - still not great.

I really believe the future will be hair cloning and implantation. Hair transplant is limited and in its infancy. It's like breast augmentation. Previous the silicone implants were horrendous. Now people can't stop looking at them.

Be patient. We receding baldies will be cured sometime in the future.

 

[yvakin]

Cool AVATAR

 

[squeegee]

It might be great news but any oligonucleotide started to degrade at room temperate. This so called innovation will have to be stored long term at 4 degree celcius.

The idea and the delivery of transport is there, but it will be very expensive to make. Creating a base pair of oligonucleotide usually costs 23pence per base pair. It will also have to be stored in water or Telogen Effluvium buffer for stability.

Lets wait before people comment on this discovery. Over the 10 years, too much hype and little progress.

Upto now, we can only trust three things:

(1) Finasteride 1mg - DHT inhibitor
(2) Minoxidil - growth stimulator
(3) Hair transplant - still not great.

I really believe the future will be hair cloning and implantation. Hair transplant is limited and in its infancy. It's like breast augmentation. Previous the silicone implants were horrendous. Now people can't stop looking at them.

Be patient. We receding baldies will be cured sometime in the future.

The future will be stem cell technology.period.

 

[toivonen]

i believe it to...meanwhile my goal is to take something (i don't care of FDA / big company stock market bull shits) that i see works in a reasnoble way, balanced with the fact that is actually making me feel goog healthy..that's why i take beta-sitosterol in diferent forms..phytosterols from pine (one of the richest sources), and flax lignans, together with goji berry juice, and a lot of green/black tea..so that way, if i don't see many "oficial" studys about theire male pattern baldness aplication, i see a lot in terms of other health problems, oxidant levels/bad cholesterol,etc...
The results from a little more than 1 year?!..i've mantained almost all of my hair, no regrowth, at least till now...but i'm entering an age where my genes are really starting to kick me down, and from what i see from other people with the same age, they've lost more hair in the last 2 years (i'm talking about 35-40 yold) than in the 5 or more years before...aging is a b**ch! :jackit: ...So i consider my results very very good..they are no less inferior to what i had i the same period of using propecia..that's a fact!
My topical has been for the last months, mainly Hairsignals from Skin Biology, wich has given me a healthy scalp condition,..at least! The topical though is the only thing i'll re-evaluate in the futur..i'll give it, 3-6 months more. Not that i don't like the product, but cause i believe topicals really are the dificult part in the problem..mantaining hasn't be my problem, but stimulating..yes! Let's see what the futur "drop" us..

Oh, the most important part about my "internal" regime..i feel and the results of medical tests proove..healthier than ever! That is waht really been the most satysfying aspect!

Good luck to you all, regardless of the "road" you choose..and let's hope that the futur will give us good/solid things to solve our little BIG problem!

 

[hairrific]

100% AR function inhibition! Now that is the topical we have been looking for.

So how many years must go by before we can finally obtain it from CHINA? :sobbing: :dunno:

We can get it now.. Just wonder what they used for the delivery agent?

I must be missing something, where can we get Antisense oligodeoxynucleotides formulated in cream preparations? The dermatologist for psoriasis maybe? Someone should start experimenting now and get this going so we can learn something.

 

[hairrific]

Wow this stuff is GREAT for preventing hair loss I can tell already!! :whistle: