An older patent showing pgd2 inhibiting hair growth.
https://www.google.com/patents/EP17...X&ei=zl7-VICWN8r_yQTar4GADQ&ved=0CBwQ6AEwADgo
HUMAN HAIR FOLLICLE GROWTH ASSAY
[0037]
Human hair follicles in growth phase (anagen) were isolated from face-lift tissue (obtained from plastic surgeons) under dissecting scope using a scalpel and watchmakers forceps. The skin was sliced into thin strips exposing 2-3 rows of follicles that could readily be dissected. Follicles were placed into 0.5 ml William's E medium (Life Technologies, Gaithersburg, MD.) supplemented with 2 mM L-glutamine, 10 µg/ml insulin, 10 ng/ml hydrocortisone, 100 units of penicillin, 0.1 mg/ml streptomycin and 0.25 µg/ml amphotericin B. The follicles were incubated in 24-well plates (1 follicle/well) at 37°C in an atmosphere of 5% CO2 and 95% air. Compounds are dissolved into dimethyl sulfoxide as 100-fold stock solution. The control hair follicles were treated with dimethyl sulfoxide without prostaglandin. The follicles were photographed in the 24-well plates under the dissecting scope at a power of 10X. Typically, image recordings were made on day 0 (day follicles were placed in culture), and again on day 7. The length of hair follicle was assessed using an image analysis software system. The growth of hair fiber was calculated by the subtracting the follicle length on day 0 from that determined on day 7.
[0038]
PGD2 and its analogs demonstrated a significant reduction of human hair follicle growth. All seven of the PGD2 analogs tested significantly reduced hair growth. The results are provided in Table III. TABLE III
REDUCTION OF HUMAN HAIR FOLLICLE GROWTH BY PGD2 AND ITS ANALOGS
Hair follicle length increase (mm)
Prostaglandin Dose (µM) Treated Control % Reduction
PGD2 30 0.24 ± 0.10 1.89 ± 0.38 87.3 ± 5.0
15-Deoxy-Δ12,14-PGD2 50 0.10 ± 0.08 1.76 ± 0.27 94.3 ± 4.5
16,16-Dimethyl PGD2 50 0.10 ± 0.05 1.76 ± 0.27 94.3 ± 2.8
15(S)-15-Methyl PGD2 100 0.08 ± 0.06 1.10 ± 0.35 92.7 ± 5.5
17-Phenyl trinor PGD2 50 0.10 ± 0.12 1.62 ± 0.43 93.8 ± 7.4
11-Deoxy-11-methylene PGD2 50 1.11 ± 0.14 1.76 ± 0.27 36.9 ± 8.0
15(R)-15-Methyl PGD2 50 0.04 ± 0.05 1.62 ± 0.43 97.5 ± 3.1
[0039]
The sequential metabolite of PGD2, namely, PGJ2 that is formed in the cells from PGD2, was also found to reduce hair growth. In addition, natural metabolites and (analogs) of PGJ2 that were tested reduced hair growth. The results are provided in Table IV. TABLE IV
Reduction of human hair follicle growth by the PGD2 metabolite, PGJ2 and its analogs
Hair follicle length increase (mm)
Prostaglandin Dose (µM) Treated Control Reduction
PGJ2 30 0.16 ± 0.25 1.89 ± 0.38 91.5 ± 13
Δ12-PGJ2 30 0.29 ± 0.46 1.89 ± 0.38 84.6 ± 24
15-Deoxy-Δ12,14-PGJ2 30 0.10 ± 0.09 1.89 ± 0.38 94.7 ± 5.0
9,10-Dihydro-15-deoxy-à 12,14-PGJ2 50 0.02 ± 0.03 1.62 ± 0.43 98.8 ± 1.9
[0040]
The hair growth inhibition profile by both PGD2 and PGJ2 (and its analogs) was found to be dose dependent. The results are provided in Table V. TABLE V
DOSE-DEPENDENT REDUCTION OF HUMAN HAIR FOLLICLE GROWTH BY PGD2 ,PGJ2 AND ITS METABOLITES
Growth of follicle (mm)
Prostaglandin Dose (µM) Treated Control % Reduction
PGD2 1 1.51 ± 0.28 1.58 ± 0.30 4.4
5 1.38 ± 0.30 1.58 ± 0.30 12.7
10 0.98 ± 0.32 1.58 ± 0.30 38.0
PGJ2 1 1.43 ± 0.34 1.58 ± 0.30 9.5
5 1.03 ± 0.35 1.58 ± 0.30 34.8
10 0.50 ± 0.29 1.58 ± 0.30 68.4
Δ-12-PGJ2 1 1.40 ± 0.44 1.41 ± 0.34 0.7
5 1.26 ± 0.38 1.41 ± 0.34 10.6
10 0.32 ± 0.15 1.41 ± 0.34 77.3
15-deoxy-Δ12,14-PGJ2 1 1.42 ± 0.37 1.41 ± 0.34 0
5 0.21 ± 0.13 1.41 ± 0.34 34.8
10 0.08 ± 0.07 1.41 ± 0.34 94.3
[0041]
Because PGD2 and PGJ2 analogs behave as agonists of the PGD2, and are expected to bind strongly to this receptor, the results indicate that this stimulation of PGD2 results in reduction of hair growth. These results further demonstrate that activation of PGD2 by a compound that acts as an agonist of PGD2 or PGJ2 results in a reduction of hair growth.
