Actual picture of the biodegradable scaffold Stemson is using for hair cloning

eeyore

Established Member
My Regimen
Reaction score
211
When comparing their latest papers published they were a little a head since the hair they created was "half" cloned (dermal papilla cells used were cloned but epithelial cells not). But both companies supposedly evolved their research past those publications so it's hard to know.

What we know is that in theory, the way Stemson is approaching the hair cloning problem is better than Tuji's, because with it they don't have the cells amplification problem that supposedly Tsuji had.

But I will only consider Stemson "officially" ahead of Tsuji when they start pre clinical studies and get positive data from that. Until then they are in reality still too early, like Tsuji was, IMO.

But of course Stemson didn't run out of money like Organ/Tsuji di (yet), in fact they appear to be well funded and they are growing their team which is a good thing. But the jury is still out.
Ah, I got from the presentation and interview that the DP cells were cloned and that they're vital, but they didn't really elaborate on epithelial cells, or how they got them out of the mice. Is it safe to assume that it's just a blood draw and that they can clone them the same as they cloned DP cells?

In the article you've linked, the abstract mentions "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." but considering that that was published nearly six years ago and that they were able to make follicles from human DP cells and transplant them into mice, is it likely this problem has been largely solved?
 

werefckd

Experienced Member
My Regimen
Reaction score
595
Ah, I got from the presentation and interview that the DP cells were cloned and that they're vital, but they didn't really elaborate on epithelial cells, or how they got them out of the mice. Is it safe to assume that it's just a blood draw and that they can clone them the same as they cloned DP cells?

In the article you've linked, the abstract mentions "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." but considering that that was published nearly six years ago and that they were able to make follicles from human DP cells and transplant them into mice, is it likely this problem has been largely solved?
Yes in Antonella's presentation she shows some drawings that indicate that the epithelial cells were derived from human (iPS) cells too, but other than that, all the other materials we have from them up to 2019 indicate they were using mice cells. But that is not really a problem IMO because since 2014 it's already known how to deriver folliculogenic epithelial stem cells from human IPS cells: https://www.nature.com/articles/ncomms4071

I guess that's why Antonella said, regarding epithelial cells, that they were "optimizing existing protocols". The thing is the novelty on their part was the discovery of deriving DP cells from iPSC so that's the part they were focusing more in the early years of their research and even nowadays give it more importance. But as of now there is no reason to believe they can't derive both kind of cells from iPSC, fundamentally the problem is solved, it's currently only a matter of optimization and "engineering" to get it perfect, according to them. Also, I don't know if you are implanting the hair gems in mice you must use mice epithelial cells? It's a possibility.

Regarding the phrase "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." -- you are right, they solved it. How they solved is by not trying to expand DP cells directly. They first expand iSPC (which is supposed to be very easy), THEN they derive DP cells from it. Same end result (you get more DP cells) but different method. Supposedly if you try to expand the DP cells themselves they will lose their properties and it won't work. This is the part where the Stemson researchers got their scientific breakthrough as nobody else had done it before. They even secured a patent related to that process.
 

jan_miezda

Banned
My Regimen
Reaction score
292
Ah, I got from the presentation and interview that the DP cells were cloned and that they're vital, but they didn't really elaborate on epithelial cells, or how they got them out of the mice. Is it safe to assume that it's just a blood draw and that they can clone them the same as they cloned DP cells?

In the article you've linked, the abstract mentions "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." but considering that that was published nearly six years ago and that they were able to make follicles from human DP cells and transplant them into mice, is it likely this problem has been largely solved?
The epithelial cells are derived from the HiPSC. Stem cell differentiation results in two classes of daughter cells (another stem cell - in this case the DP cell and a specialized cell - the epithelial cell. This is fundamental to all stem cell differentiation and is different to progenitor cell line induction
 

Attachments

  • 4F574B04-7A52-47B8-B284-8C56E75D5E9C.jpeg
    4F574B04-7A52-47B8-B284-8C56E75D5E9C.jpeg
    71.2 KB · Views: 72

jan_miezda

Banned
My Regimen
Reaction score
292
Yes in Antonella's presentation she shows some drawings that indicate that the epithelial cells were derived from human (iPS) cells too, but other than that, all the other materials we have from them up to 2019 indicate they were using mice cells. But that is not really a problem IMO because since 2014 it's already known how to deriver folliculogenic epithelial stem cells from human IPS cells: https://www.nature.com/articles/ncomms4071

I guess that's why Antonella said, regarding epithelial cells, that they were "optimizing existing protocols". The thing is the novelty on their part was the discovery of deriving DP cells from iPSC so that's the part they were focusing more in the early years of their research and even nowadays give it more importance. But as of now there is no reason to believe they can't derive both kind of cells from iPSC, fundamentally the problem is solved, it's currently only a matter of optimization and "engineering" to get it perfect, according to them. Also, I don't know if you are implanting the hair gems in mice you must use mice epithelial cells? It's a possibility.

Regarding the phrase "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." -- you are right, they solved it. How they solved is by not trying to expand DP cells directly. They first expand iSPC (which is supposed to be very easy), THEN they derive DP cells from it. Same end result (you get more DP cells) but different method. Supposedly if you try to expand the DP cells themselves they will lose their properties and it won't work. This is the part where the Stemson researchers got their scientific breakthrough as nobody else had done it before. They even secured a patent related to that process.
Where did you find that they solved it by expanding IPScells?
 

werefckd

Experienced Member
My Regimen
Reaction score
595
Where did you find that they solved it by expanding IPScells?
getting enough human iPSC is not a big deal/was never a problem. Read about it many times. For example:

Dr. Terskikh: Currently there are no methods to amplify DP cells in the dish. They loose the hair induction properties after couple of passages. This is precisely the problem. That’s why we don’t amplify DP cells in the dish. We amplify iPSCs cells, which could grow in the dish almost indefinitely. Then the “buckets” of iPSCs cells are converted to the “buckets” of DP cells that are needed for transplantation.

I think Dr. K talks about it more in detail in his YT interview from last October.

Also, you can get a lot of iPSCs from blood, so don't know even if amplification would be necessary in the first place.
 
Last edited:

eeyore

Established Member
My Regimen
Reaction score
211
Yes in Antonella's presentation she shows some drawings that indicate that the epithelial cells were derived from human (iPS) cells too, but other than that, all the other materials we have from them up to 2019 indicate they were using mice cells. But that is not really a problem IMO because since 2014 it's already known how to deriver folliculogenic epithelial stem cells from human IPS cells: https://www.nature.com/articles/ncomms4071

I guess that's why Antonella said, regarding epithelial cells, that they were "optimizing existing protocols". The thing is the novelty on their part was the discovery of deriving DP cells from iPSC so that's the part they were focusing more in the early years of their research and even nowadays give it more importance. But as of now there is no reason to believe they can't derive both kind of cells from iPSC, fundamentally the problem is solved, it's currently only a matter of optimization and "engineering" to get it perfect, according to them. Also, I don't know if you are implanting the hair gems in mice you must use mice epithelial cells? It's a possibility.

Regarding the phrase "Unfortunately human DP cells are not suitable for this purpose because they cannot be obtained in necessary amounts and rapidly loose their ability to induce hair follicle formation when cultured." -- you are right, they solved it. How they solved is by not trying to expand DP cells directly. They first expand iSPC (which is supposed to be very easy), THEN they derive DP cells from it. Same end result (you get more DP cells) but different method. Supposedly if you try to expand the DP cells themselves they will lose their properties and it won't work. This is the part where the Stemson researchers got their scientific breakthrough as nobody else had done it before. They even secured a patent related to that process.
Thanks for all the clarification and linking to other sources of information from Stemson which I've never seen before. I'm surprised I missed the interview by hairlosscure2020. It seems like money was one of their biggest hurdles to research so hopefully we can get an update within the next five years now that they have some funding.
 

Tom4362

Established Member
My Regimen
Reaction score
513

THE PROBLEM​

Regenerative Science has been a product of fiction, unobtainable in the real world, and as a society the only option we had to manage and mitigate illness and loss was within our own bodies – until now.

THE SOLUTION​

Ranked amongst the top Biotech innovations the world has ever seen, our team of scientists have spent the last 12 years working towards a major breakthrough in Stem Cell regenerative science that has global application in our daily lives. In July 2019 our team achieved this breakthrough, and is now working towards a practical application. Our certainty – the world will be changed at every level when this science is released.

DELIVERING REAL POSITIVE CHANGE​

  • Huge numbers within our global community will have significantly enhanced self-confidence and mental and physical well-being.
  • This Stem Cell technology has a large number of other applications, already identified by the team, for future investments.
  • The IP is fully protected, and the rights to commercialise the regeneration of hair follicles via Stem Cell science is completely owned by the Investee.
  • There is an opportunity to develop the world’s first Stem Cell manufacturing line in the UK.
  • Time to human clinical trials within the next 18 months.
  • Leading US pharma partner deeply involved.
So this basically confirms that they are planning to do human trials in 18 months, great
 
Last edited:

jan_miezda

Banned
My Regimen
Reaction score
292
getting enough human iPSC is not a big deal, read about it many times. I think Dr. K talks about it too in his YT interview.
But that means they will have to do multiple injections after each scaffold is planted?
getting enough human iPSC is not a big deal/was never a problem. Read about it many times. For example:

Dr. Terskikh: Currently there are no methods to amplify DP cells in the dish. They loose the hair induction properties after couple of passages. This is precisely the problem. That’s why we don’t amplify DP cells in the dish. We amplify iPSCs cells, which could grow in the dish almost indefinitely. Then the “buckets” of iPSCs cells are converted to the “buckets” of DP cells that are needed for transplantation.

I think Dr. K talks about it more in detail in his YT interview from last October.

Also, you can get a lot of iPSCs from blood, so don't know even if amplification would be necessary in the first place.
Why don’t you believe in tissuse
 

werefckd

Experienced Member
My Regimen
Reaction score
595
But that means they will have to do multiple injections after each scaffold is planted?
No. The scaffolds are filled with cells THEN implanted in the scalp. There are no injections.
Why don’t you believe in tissuse
Why should I? No studies published, not a single picture posted. Never experimented with animals. Nothing to show and the company is not researching it anymore.

If you believe THAT has a shot in solving such a hard problem that is hair cloning, then you might believe the Santa Claus is real as well.
 

Joxy

Experienced Member
Reaction score
519
This technology is at least 10-15 years away. We must live in reality, not in fantasy world. And the main question is how much it will be successful.
 

jan_miezda

Banned
My Regimen
Reaction score
292
Tissuse/ J. Hewitt
and Samumed
Same I think these will be the best options before stemson. Tissuse is delivering on their promises. They are taking their time and not rushing. I think they will start trial within 1-2 years
 

soull

Established Member
My Regimen
Reaction score
373
both of you stop crying when you are the first to post anything that contains the name "Tsuji". You can not use the forum as an informative medium, instead of complaining? Nobody knows anything about releases until otherwise published.

A word of advice for those who believe TissUse will start trials soon. Take it with caution as they have not published anything officially, so you will avoid disappointment and having to read your regrets
 
Top