Atcc Pta 6475, The Cure We Aren't Talking About?

[Unacknowledged]

Why can’t you try it out? It’s on Amazon?

Doesnt send it to my country.

 

[hailbrotzu]

Tried it for 2 months. No change in hair

 

[mercail]

Holy crap the shipping is 39.59 (To Chile) whereas the tablets (gastrus, not even osfortis) are 29$
Man screw internalization costs, this is just a ripoff

 

[asabovesobelow]

The key here is to increase the bacterial counts into the trillions and at the same time having a regular intake of prebiotics. The amount of prebiotics needed to optimally feed the bacteria is not known. An often recommended daily dose seems to range between 5 and 20g.

 

[fugged]

I tried this for a few months everyday.. then I quit, only because the whole process was a pain, heating the half and half without scalding takes forever, then cooling takes forever, mostly because my last batch I got fuzzy mold on the milk. I want to know why you can't just incubate water with prebiotic inulin in it and drink that, why the need for milk?? If we could grind up the tablets and add an appropriate amount of inulin to water in jars, and somehow figure out an incubation method (I did some in the pressure cooker, sitting in water) I wonder if that would work. Im not clear on what these bacteria eat, do they eat prebiotic fiber as they apparently do in the gut, or fat and protein in milk?? The issue is the quantity given to mice was far more than what we would get in a tablet. I dont have a microscope that could confirm the amplification is working.. couple hundred bucks.

 

[asabovesobelow]

I tried this for a few months everyday.. then I quit, only because the whole process was a pain, heating the half and half without scalding takes forever, then cooling takes forever, mostly because my last batch I got fuzzy mold on the milk. I want to know why you can't just incubate water with prebiotic inulin in it and drink that, why the need for milk?? If we could grind up the tablets and add an appropriate amount of inulin to water in jars, and somehow figure out an incubation method (I did some in the pressure cooker, sitting in water) I wonder if that would work. Im not clear on what these bacteria eat, do they eat prebiotic fiber as they apparently do in the gut, or fat and protein in milk?? The issue is the quantity given to mice was far more than what we would get in a tablet. I dont have a microscope that could confirm the amplification is working.. couple hundred bucks.

The proof for the bacteria's growth is in the thickening of the milk. Most supermarkets should have a highly pasteurized milk. They're usually labeled as lasting longer. I've always used 3% fat milk and it turns into a thick, nicely tasting, yoghurt.

The reason milk is used is because it has lactose in it which the bacteria can also feed on in addition to the prebiotic fiber or dextrose. It also tastes much better than if you'd do it with water. But if you can't find this highly pasteurized type and just find it too much of a hassle to heat and cool, then you could probably use water or another liquid without competing bacteria in it. Just have in mind that you're going to need more prebiotic fiber or dextrose to yield the same result.

 

[OtyMac]

This study went nowhere probably because of IL-10

Probiotic Bacteria Induce a ‘Glow of Health’ - PMC

Radiant skin and hair are universally recognized as indications of good health. However, this ‘glow of health’ display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary changes mimicking peak ...

pmc.ncbi.nlm.nih.gov

These data demonstrated that the probiotic-triggered features of lustrous fur required Il-10-competency in host animals.

They also figured out the probiotic essentially was acting like a IL-17 antibody:
Depletion of Il-17A using anti-cytokine antibodies recapitulates the probiotic-induced glow of health features in the skin including hair follicle anagen phase predominance and vastly increased numbers of sebocytes in sebaceous glands.

IL-17/IL10 are the keys to making this work..

see:

https://www.sciencedirect.com/science/article/pii/S2352512618300158

Not only is there a regrowth of hair which were mainly concerned about but if you hit high-res you can see his skin color has went from pale to being "more youthful"(IMO) like the mice study but he could also have been out in the sun..

 

[OtyMac]

A complementary probiotic mix which would increase IL-10 which is required for L. Reuteri would be to include any of the following which increase IL-10 expression:

How to Increase IL-10 Anti-Inflammatory Cytokine - SelfHack

While we usually think of Cytokins as bad, IL-10 is an exception. Learn how to increase it & the good & bad functions of IL-10.

selfhack.com

L. Reuteri + any or all of the below:

Probiotics: B fragilis [79], L. plantarum [80], S. boulardii [81], L casei [82], Bacillus Subtilis [83]

 

[Arthurandsam]

• Metabolites of probiotic BB536: https://www.sciencedirect.com/science/article/abs/pii/S2212429224012860

Creating a Cell-Free Supernatant (CFS) of Bifidobacterium longum BB536 at home​

Part 1: Preparing the CFS

1. 1. Prepare Growth Medium:
   • Mix 10 g sugar, 1 g yeast extract (or 1 tsp nutritional yeast), and 1 g soy protein isolate (or skim milk powder) in 1 liter of water.
   • Boil the mixture for 5 minutes to sterilize and dissolve the components.
   • Let it cool to room temperature.
2. 2. Inoculate the Medium:
   • Open a B. longum BB536 probiotic capsule and add the contents to the cooled medium.
   • Stir gently and loosely cover the container to allow gas exchange.
3. 3. Fermentation:
   • Place the jar in a warm, dark spot (~37°C if possible) for 24–48 hours. Ensure it remains undisturbed.
4. 4. Harvest the Supernatant:
   • After fermentation, strain the liquid using a coffee filter or fine mesh to remove bacterial cells.
   • Optionally, use a syringe filter (0.22 µm) to ensure sterility if available.
5. 5. Store the CFS:
   • Transfer the clear liquid into a clean bottle.
   • Store in the refrigerator if not using immediately.

Part 2: Preparing the Scalp Formulation with Propylene Glycol

1. 1. Create the Base Solution:
   • Mix 10 mL of CFS with 85 mL of distilled water.
   • Add 5 mL of propylene glycol (5% concentration is effective and safe for scalp use).
2. 2. Mix Thoroughly:
   • Stir the solution well to ensure uniform distribution of propylene glycol in the mixture.
3. 3. Check and Adjust pH (Optional):
   • Use pH strips to test the pH of the solution. Adjust to 4.5–5.5 for scalp compatibility:
     • Add a drop of diluted citric acid if the pH is too high.
     • Add a drop of baking soda solution if the pH is too low.
4. 4. Transfer to Application Bottle:
   • Pour the prepared solution into a clean dropper bottle or spray bottle for easy application.

 

[OtyMac]

Like the original poster mentioned OTC probiotics(for oral use) are way to low in CFU's to be of any benefit. The reading I've done shows the mice studies used anywhere from 10-100x what we are using.

Some of the bacteria strain manufacturers are recommending and people reporting results(non-hair related) from using 300-500 billion CFU per day. A 5-10 billion CFU Walmart probiotic isn't going to work as we've found out.