Tsuji is out of hair cloning now
he stop his research completely? until 2022
i think these concept ben proven. they just need to do overcome some barriers: (but its a new technology and its nice to see how many paper are published annually) soon it will be viable option for us
Seminal studies demonstrated that papillae isolated from rat, guinea‐pig vibrissa, but also human, could induce HF formation when implanted into a recipient non‐hairy skin.
29,
30 These experiments demonstrated that DP, unlike dermal fibroblasts, can reprogram non‐hairy epidermis to a follicular fate. Later on, several groups demonstrated hair regeneration in immunodeficient mice skin when combining different populations of dissociated cells,
31 including DP and DS,
32 but also freshly isolated epithelial bulge cells.
33 More recently, ablation of DPCs unequivocally demonstrated its pivotal role on hair growth but not on epithelial HF maintenance.
13 Moreover, the generation of rodent pelage by transplantation of dissociated DPCs has been possible in a variety of different approaches, as revised in Ohyama et al.
34 However, inducing human HF‐like structures in nude mice only proved possible when human DPCs were combined with epidermal component,
35-
37 clearly indicating the need for EM interactions. Thus, a successful bioengineering solution will require an available amount of competent epithelial and inductive DPCs.
culturing cell is issue for haircloning (this is not from him cells but I think similar problem occur):
DP in vitro cell expansion is inevitable toward the development of a clinically relevant tissue engineering‐based solution for HF regeneration. However, two major technical burdens have limited the attainment of human trichogenic DPCs. In contrast to mouse, isolation of human DPCs requires DP microdissection from HF punches, as enzymatic digestion with trypsin and collagenase inefficiently works to generate single cells for FACS‐sorting, and robust cell surface markers (as, eg, CD133 in the mouse) remain to be defined.
34 Moreover, enzymatic digestion would deprive DP of its natural and distinctive extracellular matrix microenvironment, which is essential for hair inductive properties. This is also a caveat of in vitro expansion, particularly under regular culture conditions lacking key environmental cues.
38,
39 Considering the relevance of DP for HF cycle, massive effort has been made on finding out an in vitro expansion procedure for human DPCs that preserves, or alternatively rescues, their hair inductive ability.
