Pgd2 ?how Can I Do It?help Me Help Me

[pegasus2]

Woops forgot to reply, yeah they linked a few SNPs to the responsiveness to pgd2 but haven't fine tuned exactly how. Might give an indication but definitely not an accurate prediction if you've had your genes sequenced.

If you've done a test, check any of these:
rs545659 - CT or CC => likely PGD2-sensitive, TT => likely PGD2-insensitive
rs634681 - AG or AA => likely PGD2-sensitive, GG => likely PGD2-insensitive
rs7167 - AG or GG => likely PGD2-sensitive, AA => likely PGD2-insensitive

TRANSLATION:
likely PGD2-sensitive => 64.1% chance of PGD2 sensitivity
likely PGD2-insensitive => 36.5% chance of PGD2 sensitivity

Thanks. I was reading the patent this morning, and I am unlikely to be a responder. I don't know about the third, but on the first two I'm homozygous for the major allele.

It's good to see you around again. While you're here, what do you think about the latest Follica patent? They are coming back again to EGFR inhibition after wounding. EGF is important for HF cyclying. Do you think it's a good idea to inhibit it for 7-10 days post-wounding while morphogenesis occurs?

 

[Fgsfds]

If you've done a test, check any of these:
rs545659 - CT or CC => likely PGD2-sensitive, TT => likely PGD2-insensitive
rs634681 - AG or AA => likely PGD2-sensitive, GG => likely PGD2-insensitive
rs7167 - AG or GG => likely PGD2-sensitive, AA => likely PGD2-insensitive

TRANSLATION:
likely PGD2-sensitive => 64.1% chance of PGD2 sensitivity
likely PGD2-insensitive => 36.5% chance of PGD2 sensitivity

I browsed my raw data on 23ndme, and:

rs545659: don't have
rs634681: GG
rs7167: don't have

Is it possible to "not have" a SNP? Aren't there just positions on a chromosome, therefore "not having" a SNP is a nonsensical statement? Did 23andme just not sequence it?

How do those 3 variables play together? Is it implied that if one suggests PGD2 insensitive, the other 2 will imply such as well? What if one has "contradicting" SNPs?

 

[John Difool]

I browsed my raw data on 23ndme, and:

rs545659: don't have
rs634681: GG
rs7167: don't have

Is it possible to "not have" a SNP? Aren't there just positions on a chromosome, therefore "not having" a SNP is a nonsensical statement? Did 23andme just not sequence it?

How do those 3 variables play together? Is it implied that if one suggests PGD2 insensitive, the other 2 will imply such as well? What if one has "contradicting" SNPs?

Which package on 23andme let you see these?

 

[John Difool]

Worth inquiring to them

 

[InBeforeTheCure]

Thanks. I was reading the patent this morning, and I am unlikely to be a responder. I don't know about the third, but on the first two I'm homozygous for the major allele.

It's good to see you around again. While you're here, what do you think about the latest Follica patent? They are coming back again to EGFR inhibition after wounding. EGF is important for HF cyclying. Do you think it's a good idea to inhibit it for 7-10 days post-wounding while morphogenesis occurs?

This one? Interesting. EGF blocks embryonic hair follicle formation at the expense of normal skin cells, so not surprising it also does the same after wounding. I wonder about a wounding + EGFR inhibitor + Wnt agonist + (other things?) combo...But would 7-10 days be enough?

I browsed my raw data on 23ndme, and:

rs545659: don't have
rs634681: GG
rs7167: don't have

Is it possible to "not have" a SNP? Aren't there just positions on a chromosome, therefore "not having" a SNP is a nonsensical statement? Did 23andme just not sequence it?

Well, it seems like you're not fully human.

Seriously though, probably just a no call.

How do those 3 variables play together? Is it implied that if one suggests PGD2 insensitive, the other 2 will imply such as well? What if one has "contradicting" SNPs?

The three are perfectly correlated (or at least nearly so). Once you know one, the rest are redundant.

 

[Fgsfds]

This one? Interesting. EGF blocks embryonic hair follicle formation at the expense of normal skin cells, so not surprising it also does the same after wounding. I wonder about a wounding + EGFR inhibitor + Wnt agonist + (other things?) combo...But would 7-10 days be enough?



Well, it seems like you're not fully human.

Seriously though, probably just a no call.



The three are perfectly correlated (or at least nearly so). Once you know one, the rest are redundant.

High IQ, thanks mate

 

[Fgsfds]

Also @InBeforeTheCure, where did you get your numbers:

TRANSLATION:
likely PGD2-sensitive => 64.1% chance of PGD2 sensitivity
likely PGD2-insensitive => 36.5% chance of PGD2 sensitivity

Here are my numbers:

75% is the correct positive rate. For completeness, one should also compute the false negative rate (which is P(G | A^C)), but I'm lazy, and therefore leaving that as an exercise for the reader

 

[InBeforeTheCure]

Also @InBeforeTheCure, where did you get your numbers:

The wrong place! I took the allele frequencies from one of the other groups.

Here are my numbers:

View attachment 140433

75% is the correct positive rate. For completeness, one should also compute the false negative rate (which is P(G | A^C)), but I'm lazy, and therefore leaving that as an exercise for the reader

75% and 0.3077*0.52/0.52 = 30.77%. The motivated and (rightly) skeptical reader is left to check the latter.

 

[pegasus2]

This one? Interesting. EGF blocks embryonic hair follicle formation at the expense of normal skin cells, so not surprising it also does the same after wounding. I wonder about a wounding + EGFR inhibitor + Wnt agonist + (other things?) combo...But would 7-10 days be enough?

That's the one.

Good question. It might not be enough. I hesitate to go much longer, maybe up to two weeks when we know the hf placode is formed.

"Disruption or blocking of the EGF receptor (EGFR) in several mouse models results in abnormalities occurring late on in follicle development and during the adult hair follicle cycle, leading to the hypothesis that EGF signalling has an important positive influence on follicle development and growth"
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729337/

It only needs to be disrupted during placode formation. After that it has to be turned on again. It looks like Cotsarelis and Ito think 3-12 days. I wish we had something other than mouse models to go by.


The inhibitory action by EGF was reversible and stage specific, i.e., at an early stage of the development of hair rudiments.
https://www.ncbi.nlm.nih.gov/pubmed/9281334/

I'm looking at going all in, adding spironolactone+estradiol to dutasteride, RU, and topical estriol. Then wounding + single dose shh agonist + EGFR inhibitor x days? + WAY-316606, and stemoxydine for hypoxia. Maybe add some PGE2 also. If this doesn't grow hair nothing will.

 

[John Difool]

Maybe Bica over spironolactone? What EGFR inhibitors are you thinking?

 

[pegasus2]

Maybe tyrphostin.

I think it's harder on the liver. Lower AR affinity than spironolactone, but it seems like it might give more regrowth. I don't know why. Maybe because it increases estrogen significantly whereas spironolactone may or may not increase it. It is definitely more feminizing than spironolactone, with higher risks of gyno. If I use spironolactone it will only be a small dose(50mg). Depending on your situation you may want to go with bica or use a higher dose of spironolactone. My hair is stabilized and I already have vellus hairs growing on my hair line so in actuality another AA might be overkill. My AR sensitivity is low so dutasteride and RU is probably all I need to keep androgen activity below the threshold required for repair. The piece I'm missing is activating the pathways that are shut down so that they can repair the damage, which is what estradiol and everything else is for. Your case may be different. If you have moderate to high AR sensitivity, and/or you aren't getting regrowth as is, then you'll likely want to hit the AR angle harder and use bicalutamide or 150-200mg spironolactone if you can tolerate the sides.

 

[John Difool]

For regrowth without wounding the only way seems tto be he Shh path

 

[killDHT]

like this

 

[killDHT]

I saw a very powerful regenerative man, he did not use other drugs, he used lactoferrin twice a day, what do you think? And I thought women also reported rebirth.

 

[John Difool]

like this

Where is this coming from?

 

[pegasus2]

https://www.ncbi.nlm.nih.gov/pubmed/31006055

 

[John Difool]

I used 0.5%. You used 0.1%. We can compare progress.

 

[killDHT]

lol This guy lost substantial ground. The second pic is his before pic. The first pic is a month later. He is trying to avoid proven treatments and receding hard.

What are you talking about? Bring the evidence.

 

[John Difool]

lol This guy lost substantial ground. The second pic is his before pic. The first pic is a month later. He is trying to avoid proven treatments and receding hard.

I hate when the after pics look worse than the before ones.

 

[killDHT]

I hate when the after pics look worse than the before ones.

Show you this. His hair has improved, OK?