AM Nelson,[SUP]1[/SUP] DE Loy,[SUP]1[/SUP] JA Lawson,[SUP]2,3[/SUP] AS Katseff,[SUP]1[/SUP] GA FitzGerald[SUP]2,3[/SUP] and LA Garza[SUP]1[/SUP]
1 Dermatology, Johns Hopkins University School of Medicine, Baltimore, MD, 2 Pharmacology, University of Pennsylvania, Philadelphia, PA and 3 Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, PA
Prostaglandins (PGs) are key inflammatory mediators involved in wound healing. Recent evidence also illustrated a role for PGs in skin homeostasis with a specific role in regulating hair growth; however, no study to date has examined their role in skin regeneration after injury. Using the
wound-induced hair follicle neogenesis (WIHN) assay, we hypothesized that PGs would impact hair follicle neogenesis, a marker for skin regeneration. We found that in C57Bl/6J mice, the levels of prostaglandin synthases and their products (PGD2, PGE2, PGF2a) were reciprocally expressed during wound healing.
PGE2 and PGF2a levels increased during the early phases of wound healing while PGD2 increased during the later stages of wound healing (n>3; p<0.05). The level of hair follicle neogenesis after wounding varied significantly among C57Bl/6J, FVB/N and mixed background strains of mice (n>33; p<0.01). Levels of lipocalin-type prostaglandin D2 synthase (Ptgds) mRNA (n>4; p<0.01) and its product PGD2 (n=6; p<0.05) inversely correlated with regeneration ability.
C57Bl/6 mice had the highest level of Ptgds mRNA expression, PGD2 levels and the least follicle neogenesis. Additionally, an alternatively spliced transcript variant of Ptgds missing exon 3 correlated with the regeneration phenotype among strains of mice (n=9; p<0.05). Exogenous application of PGD2 decreased WIHN in wild type mice (n=8; p=0.06). Of the two canonical PGD2 receptor null mice, only Gpr44 showed increased WIHN compared to strain-matched control mice (n=31; p<0.05). In all, these findings demonstrate that PGD2 inversely correlates with hair follicle regeneration implying
that inhibition of PGD2 production or Gpr44 signaling pathways may promote skin regeneration
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3593761/figure/F2/
if you look at this picture ,it shows that PGD2 inhibition is more important than increasing pge2 levels.
the mice with lowest pgd had most hair neogenesis (not that she doesnt have the most pge2 levels).
Also how often should i apply it? I hear is best to not apply anything after derma rolling