Tsuji - News Successfully Developed An Expansion Method Of Hf Derived Stem Cells

[Endmymisery]

"To achieve hair follicle regeneration at hair densities of 120 or 60–100 hair shafts cm−² of normal scalp or in FUT treatment, respectively, twenty-eight bioengineered hair germs were transplanted into a cervical skin circle with a diameter of 1 cm. At 14 to 21 days after transplantation, the bioengineered hairs were erupted at a high density of 124.0±17.3 hair shafts cm−² (n=3; Fig 2c)"

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Any desired density is achievable. The experiment was done 3 times, and every hair that was transplanted onto the mouse grew. And as most people already know, Riken found out how to control hair color and thickness as well, so you could essentially buy yourself a scalp full of hair that is thicker than it is supposed to be. Although I suspect this will only work with NW7, who have very little hair and can therefore mask their original hairs with the much thicker ones better.

Source:
https://www.nature.com/articles/ncomms1784

I want to hope..

 

[forlorn]

107-142 hairs/cm² is really a dream.

Does somebody know if there is a survival possibility for hairs that have been transplanted back into the donor?

I don't think they have done it so far. This is what they're supposed to do in 2018. But it is my impression that the survival rate would be very high given the following findings:

- Human hair follicle transplantion into mice was successful at high density
- No interspecific transplant rejection: which means that logically, the odds of this experiment being replicated more successfully in a human-to-human transplant is higher

 

[MrV88]

I don't think they have done it so far. This is what they're supposed to do in 2018. But it is my impression that the survival rate would be very high given the following findings:

- Human hair follicle transplantion into mice was successful at high density
- No interspecific transplant rejection: which means that logically, the odds of this experiment being replicated more successfully in a human-to-human transplant is higher

Mice to human should be more difficult like you said. Human-to-human "should" be way easier, if not I have to order about 50-100 mice for growing my own grafts lol.

The best thing until now about Tsujis work is that he implemented human cells and not just grew mice hair in mice.

As far as I remember Tsuji stated that scarred areas couldn't be done now, but will be in their focus after releasing their treatment.

 

[forlorn]

Mice to human should be more difficult like you said. Human-to-human "should" be way easier, if not I have to order about 50-100 mice for growing my own grafts lol.

The best thing until now about Tsujis work is that he implemented human cells and not just grew mice hair in mice.

As far as I remember Tsuji stated that scarred areas couldn't be done now, but will be in their focus after releasing their treatment.

Overcoming the challenge of transplanting hair follicles onto scarred surfaces will certainly be far easier than mastering the cloning of hair follicles.

I believe that one of the main reasons behind Riken's rapid progression - and consequently their ambitious release date of 2020 - is that they succeeded in transplanting human hair follicles onto a mouse with a very high survival rate, despite the possiblity of transplant rejection as a result of major genetic differences. This means that they've been spared potentially years worth of work due to the high biological compatibility of their technique. Otherwise, they would've been forced to clone mouse hair, transplant it onto mice, and then continue on to human cloning and transplanting. In other words, additional time-consuming stages of research.

The best thing about Riken and Tsuji, as far as I'm concerned, is that they have unlimited funding, unlike all the other pipeline companies. Dr. Tsuji is a well-known scientist in Japan, and the fact that his research is being funded by the Japanese government makes his work a lot easier.

 

[MrV88]

Overcoming the challenge of transplanting hair follicles onto scarred surfaces will certainly be far easier than mastering the cloning of hair follicles.

I believe that one of the main reasons behind Riken's rapid progression - and consequently their ambitious release date of 2020 - is that they succeeded in transplanting human hair follicles onto a mouse with a very high survival rate, despite the possiblity of transplant rejection as a result of major genetic differences. This means that they've been spared potentially years worth of work due to the high biological compatibility of their technique. Otherwise, they would've been forced to clone mouse hair, transplant it onto mice, and then continue on to human cloning and transplanting. In other words, additional time-consuming stages of research.

The best thing about Riken and Tsuji, as far as I'm concerned, is that they have unlimited funding, unlike all the other pipeline companies. Dr. Tsuji is a well-known scientist in Japan, and the fact that his research is being funded by the Japanese government makes his work a lot easier.

So potentially this could also help people that have a lot of scars, or have already done a FUE/FUT/depleted donors in the near future?

I really want to know what procedures will be done by Riken (automated processes) and if later surgeons won't be necessary anymore.

 

[forlorn]

So potentially this could also help people that have a lot of scars, or have already done a FUE/FUT/depleted donors in the near future?

I really want to know what procedures will be done by Riken (automated processes) and if later surgeons won't be necessary anymore.

Here's what Riken has to say about surgeons and automated processes:

"So, at the initial phase, we plan to use only the surgeons having the level of skills which meets our criteria in order to enables us to ensure stable effectivity and safety. Eventually, in the future, we plan to transfer our technologies to surgeons at clinics in order to increase the number of certified surgeons certified in order to have our technology widely used. As for the last question of automating the transplanting process, as Toyoshima has touched on this earlier, we think that automating the transplanting process is an effective means to ensure stable effects. Therefore, we would like to consider developing a device to automate follicle transplants in the future."

[...]

"We believe that in order to provide our technologies to a broader range of people, we will eventually need to automate the process using a device. However, the current level of the skills owned by surgeons specialized in hair transplants is excellent, generating great results in treatments of alopecia patients. Therefore, we would like to leverage on such skills of surgeons as much as possible. Eventually, these skills are accumulated resulting in certain processes. Therefore, we believe that it is appropriate to develop automation devices incorporating such processes."

Here's what they have to say about scars:

"With our hair regeneration therapy using the hair follicle germ regeneration technology, we can artificially combine cellular tissues to tailor to the specific needs of the patient. Therefore, we believe in principle, that even for a patient with various special clinical requirements such as scars, we will be able provide a hair regeneration therapy for these individuals in the future, which is carefully tailored to such needs."

What does this mean? Well, for starters, it means that they don't know whether they can transplant hair over scar-ridden areas simply because they haven't tried it yet.

As for people who have had multiple FUT/FUE-transplants and depleted their donor area: it doesn't matter. You still have donor hair, it's just that it's on your scalp now. You still have skin in the original donor area. They can combine the skin of the donor area with the donor hair on your scalp in order to cultivate hair follicles. Although they're implying that skin won't be necessary:

"However, follicles have a characteristic which enables them to grow through normal hair cycles by interacting with the surrounding tissue."

Source:
https://www.hairlosstalk.com/news/new-research/hair-primordiums-tsuji-organ-interview-sept2016/

 

[MrV88]

What does this mean? Well, for starters, it means that they don't know whether they can transplant hair over scar-ridden areas simply because they haven't tried it yet.

As for people who have had multiple FUT/FUE-transplants and depleted their donor area: it doesn't matter. You still have donor hair, it's just that it's on your scalp now. You still have skin in the original donor area. They can combine the skin of the donor area with the donor hair on your scalp in order to cultivate hair follicles. Although they're implying that skin won't be necessary:

Waiting for the day that they are going to try this in human and scarred areas.

Thanks a lot

 

[Trichosan]

Waiting for the day that they are going to try this in human and scarred areas.

Thanks a lot

Selling my house and liquidating assets now for 500 cm2 Tsuji implantation.

 

[MrV88]

Selling my house and liquidating assets now for 500 cm2 Tsuji implantation.

LOL...wait 2-3 years xD

 

[Trichosan]

a

Here's what Riken has to say about surgeons and automated processes:

"So, at the initial phase, we plan to use only the surgeons having the level of skills which meets our criteria in order to enables us to ensure stable effectivity and safety. Eventually, in the future, we plan to transfer our technologies to surgeons at clinics in order to increase the number of certified surgeons certified in order to have our technology widely used. As for the last question of automating the transplanting process, as Toyoshima has touched on this earlier, we think that automating the transplanting process is an effective means to ensure stable effects. Therefore, we would like to consider developing a device to automate follicle transplants in the future."

[...]

"We believe that in order to provide our technologies to a broader range of people, we will eventually need to automate the process using a device. However, the current level of the skills owned by surgeons specialized in hair transplants is excellent, generating great results in treatments of alopecia patients. Therefore, we would like to leverage on such skills of surgeons as much as possible. Eventually, these skills are accumulated resulting in certain processes. Therefore, we believe that it is appropriate to develop automation devices incorporating such processes."

Here's what they have to say about scars:

"With our hair regeneration therapy using the hair follicle germ regeneration technology, we can artificially combine cellular tissues to tailor to the specific needs of the patient. Therefore, we believe in principle, that even for a patient with various special clinical requirements such as scars, we will be able provide a hair regeneration therapy for these individuals in the future, which is carefully tailored to such needs."

What does this mean? Well, for starters, it means that they don't know whether they can transplant hair over scar-ridden areas simply because they haven't tried it yet.

As for people who have had multiple FUT/FUE-transplants and depleted their donor area: it doesn't matter. You still have donor hair, it's just that it's on your scalp now. You still have skin in the original donor area. They can combine the skin of the donor area with the donor hair on your scalp in order to cultivate hair follicles. Although they're implying that skin won't be necessary:

"However, follicles have a characteristic which enables them to grow through normal hair cycles by interacting with the surrounding tissue."

Source:
https://www.hairlosstalk.com/news/new-research/hair-primordiums-tsuji-organ-interview-sept2016/

This is encouraging because I believe there is a driving force now that was uncertain before. They have invested in significant infrastructure for research and production. They have important alliances that are well financed. Their intellectual and research efforts are focused and goal oriented, they believe they "see the light at the end of the tunnel." And this:

"...Mr. Toyoshima: Yes, as I mentioned earlier, we consider the development of the technology to culture epithelial stem cells as the most significant issue we face..."

Overcoming this, which is likely, would probably generate a patent. Another commercial plus.
If there is any increment of drive like Jobs pushed at Apple, things will become reality faster then we may expect.

 

[sadila]

a


This is encouraging because I believe there is a driving force now that was uncertain before. They have invested in significant infrastructure for research and production. They have important alliances that are well financed. Their intellectual and research efforts are focused and goal oriented, they believe they "see the light at the end of the tunnel." And this:

"...Mr. Toyoshima: Yes, as I mentioned earlier, we consider the development of the technology to culture epithelial stem cells as the most significant issue we face..."

Overcoming this, which is likely, would probably generate a patent. Another commercial plus.
If there is any increment of drive like Jobs pushed at Apple, things will become reality faster then we may expect.

"the development of the technology to culture epithelial stem cells" can you clarify what he means by this ?

is it related to the problem of "mechanizing" the whole process ? or is he talking about the conductivity issue ?

 

[That Guy]

You are correct, they transplanted human hair follicles onto a mouse. The hair follicles grew successfully, and considering that they made human hair follicles grow using a mouse (meaning the method is compatible between species), it would certainly explain why they're setting the commercial release date as early as 2020.

"Painstaking research began with the bioengineering of various types of hair-follicle germs, which were grafted onto bald mice. The germs carried tiny nylon guides to nudge the growing hairs in the right direction.

Once that proved successful, the team moved on to bioengineering human hair-follicle germs and transplanting them in mice.

The human hairs grew in 21 days with the correct shafts, inner root sheaths and surrounding cells that normal human hair would, the study said.

Further tests proved the human hairs were, indeed, human."

Sources:
https://www.nature.com/articles/ncomms1784
https://www.thestar.com/news/world/...ese_scientists_regrow_human_hair_in_mice.html

@NewUser , like I was saying...

 

[Trichosan]

"the development of the technology to culture epithelial stem cells" can you clarify what he means by this ?

is it related to the problem of "mechanizing" the whole process ? or is he talking about the conductivity issue ?

They lack a clear understanding of how to perpetuate epithelial stem cells in culture. Not a matter of production technique.

 

[sadila]

They lack a clear understanding of how to perpetuate epithelial stem cells in culture. Not a matter of production technique.

So same issue that has been around for 15 years

 

[Hate da Bt]

So same issue that has been around for 15 years

May you glance at the heading of this thread?
Tsuji's team has been trying to expand mesenchymal and epithelial stem cells for quite a long time.
They have successfully expanded mesenchymal (DP) cells, and in this thread @Noisette let us know that they may have successfully expanded epithelial stem cells, as well.
They can't proceed with the human trials, if they don't achieve adequate human stem cell expansion.

 

[H]

Why is anybody waiting for Follica to come out? The wounding technique can already be done is being done by some people why not just start your own dhurat regime if your waiting for Follica? Do they have a super secret sauce that is worth waiting on that is better? Has the device's method been proven superior to regular wounding with a roller? Do they have a chance to capitalize on the wounding method because nobody else has? Is it because you believe in Puretech, for their other products they've released from breakthroughs have been very good? If so, what products are those and why did you like them?

 

[MrV88]

Why is anybody waiting for Follica to come out? The wounding technique can already be done is being done by some people why not just start your own dhurat regime if your waiting for Follica? Do they have a super secret sauce that is worth waiting on that is better? Has the device's method been proven superior to regular wounding with a roller? Do they have a chance to capitalize on the wounding method because nobody else has? Is it because you believe in Puretech, for their other products they've released from breakthroughs have been very good? If so, what products are those and why did you like them?

Seems like Follica is the only one that surely knows how deep and how often you have to treat your bald shinning bowling ball.
And a lot of us have problems of getting the right compounds together at the right dosage for a reasonable price.

 

[H]

Seems like Follica is the only one that surely knows how deep and how often you have to treat your bald shinning bowling ball.
And a lot of us have problems of getting the right compounds together at the right dosage for a reasonable price.

I see

 

[razzmatazz91]

Seems like Follica is the only one that surely knows how deep and how often you have to treat your bald shinning bowling ball.
And a lot of us have problems of getting the right compounds together at the right dosage for a reasonable price.

What are these compounds you speak of? RU?

 

[MrV88]

What are these compounds you speak of? RU?

Wasn't Royaume or That Guy the specialist?
FDG9, minoxidil and several things. You have to go through the thread buddy, don't know them one by one. Noisette posted some of the patents too.