Right now there are 3 approaches:
1. Follow the original derma roller study, this is the simplest. One thing we should ask is, did they induce follicular neogenesis in that trial, or did they somehow revive dormant existing follicle through the 1.5mm rolling + minoxidil when they stated that new hair growth was noticed at 6 weeks. I highly suspect it was existing miniaturized comatosed follicle that sprung back to life. In any case, this is by far the easiest approach to follow
2. Follow the gory wounding approach, the perfect example for this is the BBQ old man. This is also stated in the 2012 follica patent, notably, removing portions of the scalp at 5-7mm depth, by completing scraping off the epidermis, dermis and sub-dermis. A dermaroller cannot achieve this, period. Only a scalpel or worse, falling asleep head first into a hot george foreman grill
3. This is what some of the crazy mad scientist wanna be like myself are trying to achieve. This is based on the latest follica discovery of the fgf9 link. For this to occur, you need to somehow induce fgf9 at a given stage of wound healing, and bam, new hair. Since there is a complexity and somewhat bio-hazard uncertainty about buying fgf9 from some random guy in china, we think it is possible to induce fgf9 through PGE2, because PGE2 induces FGF9. Minoxidil also induces PGE2. That said, from my personal research, this is a quite difficult to achieve, the reason is because it is dose dependent and time dependent. For one, I will go ahead and say that this
cannot be achieved by minoxidil, the concentration of PGE2 necessary for this is just too high.
http://mcb.asm.org/content/26/22/8281.full.pdf
To investigate the effects of PGE2 on FGF-9 expression, primary culture human endometriotic stromal cells weretreated with various doses of PGE2 (0.01 to 100 uM) and concentrations of mRNA were quantified using quantitativeRT-PCR (see Fig. S1A and B in the supplemental material).The result demonstrated that PGE2 induced FGF-9 mRNAexpression in dose- and time-dependent manners (Fig. 1A andB). The expression of FGF-9 mRNA was induced by 0.1, 1, and10 uM PGE2, whereas higher concentrations of PGE2 failed toexert such effect. Administration of cells with 1 uM PGE2 enhanced FGF-9 mRNA expression at 8 h; expression reached a maximum at 12 h and then declined toward the basal level at24 h after PGE2 treatment. The induction of FGF-9 mRNA byPGE2 was mirrored by the increase in FGF-9 protein (Fig. 1C).
So basically they treated with different dosages between 0.01uM and 100uM and found that it is dose dependent and the lowest concentration to be 0.1uM and highest concentration 10uM. Therefore if you need to induce fgf9 through the PGE2 pathway you need a source of PGE2 that falls right in between those two number.
incidentally, PGE2 having a molecular weight of 352g/M, the dosage 23uM/ml of PGE2 in semen falls within the FGF9 inducing margin.... just saying :whistle:
